PARISS micro-spectrophotometer wavelength
calibration spectrum from 365 to 920 nm
The Full Width at Half Maximum of
the Hg 405 nm doublet is 1 nm
The wavelength dispersion varies with the refractive
index of glass,and therefore spectral resolution.
Prisms present highest spectral resolution in the
UV and blue, and lowest resolution in the red
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Spectral range: 365 - 920 nm simultaneously
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Spectral resolution ~1 nm at 436 nm
•
Transmission: Prism micro-spectrophotometers
transmit up to 100% of all wavelengths above 390 nm to
provide sensitivity that exceeds most, if not all
diffraction gratings. A prism completely eliminates the
problems associated with higher order diffraction that
are always present with diffraction gratings
View this movie to see how diffraction
gratings produce high order diffraction
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