The PARISS® Micro-Spectrophotometer The Spectral Characteristics of The PARISS Micro-Spectrophotometer High resolution spectrum taken with PARISS Micro-spectrophotometer PARISS micro-spectrophotometer wavelength calibration spectrum from 365 to 920 nm Full Width at Half Maximum resolution presented by the PARISS Micro-spectrophotometer The Full Width at Half Maximum of the Hg 405 nm doublet is 1 nm The wavelength dispersion varies with the refractive index of glass,and therefore spectral resolution.  Prisms present highest spectral resolution in the UV and blue, and lowest resolution in the red Spectral range: 365 - 920 nm simultaneously Spectral resolution ~1 nm at 436 nm Transmission: Prism micro-spectrophotometers transmit up to 100% of all wavelengths above 390 nm to provide sensitivity that exceeds most, if not all diffraction gratings.  A prism completely eliminates the problems associated with higher order diffraction that are always present with diffraction gratings View this movie to see how diffraction gratings produce high order diffraction  LightForm_Logo Copyright © LightForm Inc, 2011 Prism resolution versus refractive index